Shawn sent this over. Apparently at least ten years old. A kid gets busted for possession in Florida and his chemistry professor father goes to work. Figures out how to splice thc onto oranges. Wonder how hard the technology really is?
“It’s quite simple, really,” Nanofsky explains, “I wanted to combine Citrus sinesis with Delta 9-tetrahydrocannabinol.” In layman’s terms, the respected college professor proposed to grow oranges that would contain THC, the active ingredient in marijuana. Fourteen years later, that project is complete, and Nanofsky has succeeded where his letter writing campaign of yore failed: he has the undivided attention of the nation’s top drug enforcement agencies, political figures, and media outlets. The turning point in the Nanofsky saga came when the straight-laced professor posted a message to Internet newsgroups announcing that he was offering “cannabis-equivalent orange tree seeds” at no cost via the
mail. Several weeks later, U.S. Justice Department officials showed up at the mailing address used in the Internet announcement: a tiny office on the second floor of the Dittmer Laboratory of Chemistry building on the FSU campus. There they would wait for another 40 minutes before Prof. Nanofsky finished delivering a lecture to graduate students on his recent research into the “cis-trans photoisomerization of olefins.” U.S.
“I knew it was only a matter of time before someone sent me more than just a self-addressed stamped envelope,” Nanofsky quips, “but I was surprised to see Janet Reno’s special assistant at my door.” After a series of closed door discussions, Nanofsky agreed to cease distribution of the THC-orange seeds until the legal status of the possibly narcotic plant species is established.
Much to the chagrin of authorities, the effort to regulate Nanofsky’s invention may be too little too late. Several hundred packets containing 40 to 50 seeds each have already been sent to those who’ve requested them, and Nanofsky is not obliged to produce his mailing records. Under current law, no crime has been committed and it is unlikely that charges will be brought against the fruit’s inventor.
Now it is federal authorities who must confront the nation’s unwieldy body of inconsistent drug laws. According to a source at the Drug Enforcement Agency, it may be months if not years before all the issues involved are sorted out, leaving a gaping hole in
drug policy in the meantime. At the heart of the confusion is the fact that THC now naturally occurs in a new species of citrus fruit. U.S.
As policy analysts and hemp advocates alike have been quick to point out, the apparent legality (for now) of Nanofsky’s “pot orange” may render debates over the legalization of marijuana moot. In fact,
’s top law enforcement officials admit that even if the cultivation of Nanofsky’s orange were to be outlawed, it would be exceedingly difficult to identify the presence of outlawed fruit among the state’s largest agricultural crop. Florida
Amidst all of the hubbub surrounding his father’s experiment, Irwin Nanofsky exudes calm indifference. Now 30-years-old and a successful environmental photographer, the younger Nanofsky can’t understand what all of the fuss is about. “My dad’s a chemist. He makes polymers. I doubt it ever crossed his mind that as a result of his work tomorrow’s kids will be able to get high off of half an orange.”
Biochem 101: How to design a Cannabis-equivalent citrus plant
Biochemically isolate all the required enzymes for the production of THC.
Perform N-terminal sequencing on isolated enzymes, design degenerate PCR (polymerase chain reaction) primers and amplify the genes.
Clone genes into an agrobacterial vector by introducing the desired piece of DNA into a plasmid containing a transfer or T-DNA. The mixture is transformed into Agrobacterium tumefaciens, a gram negative bacterium.
Use the Agrobacterium tumefaciens to infect citrus plants after wounding. The transfer DNA will proceed to host cells by a mechanism similar to conjugation. The DNA is randomly integrated into the host genome and will be inherited.
This technology could really help the sagging Fallbrook citrus industry. A link to another article here. Takes care of scurvy as well. And no more problems getting the kids to finish their juice.